Hotspot

petA-psbJ

LSC · 61680-62495 · 816 bp

Back to Achnatherum

Pi 0.0123

MarkerSeek score 62.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 15
pi: 0.0123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTCCTGTGATTGGT	50.0	47.6	57.9	58.0	993 bp	1.0	72.8
petA-psbJ_p2	TGTTCTTCTTCGCATCCGTT	GCTGGTATTCCTGTGATTGGT	45.0	47.6	57.8	58.0	986 bp	1.0	71.9
petA-psbJ_p3	TTGTTCTTCTTCGCATCCGT	GCTGGTATTCCTGTGATTGGT	45.0	47.6	57.8	58.0	987 bp	1.0	71.9
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	AGCTGGTATTCCTGTGATTGG	50.0	47.6	57.9	57.7	994 bp	1.0	70.5
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGGCTGATAGGTACTGTAGCT	50.0	47.6	57.9	57.7	1011 bp	1.0	70.3