Hotspot

rpoB-rpoC1

LSC · 23558-23594 · 37 bp

Back to Achnatherum

Pi 0.036

MarkerSeek score 63.00

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 37
variable_sites: 2
pi: 0.036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	CGTGCGAGAACTACGATCTT	CGCCAACGACTTCTCCATTA	50.0	50.0	57.8	58.0	231 bp	1.0	79.3
rpoB-rpoC1_p2	CGTGCGAGAACTACGATCTT	TTTTGTTAGCCCAAGCCCTT	50.0	45.0	57.8	57.9	204 bp	1.0	78.6
rpoB-rpoC1_p3	CGTGCGAGAACTACGATCTT	TTTTTGTTAGCCCAAGCCCT	50.0	45.0	57.8	57.9	205 bp	1.0	78.6
rpoB-rpoC1_p4	CTCGTGCGAGAACTACGATC	CGCCAACGACTTCTCCATTA	55.0	50.0	58.3	58.0	233 bp	1.0	78.5
rpoB-rpoC1_p5	CGTGCGAGAACTACGATCTT	GTTTTTGTTAGCCCAAGCCC	50.0	50.0	57.8	57.8	206 bp	1.0	78.1