Hotspot

trnC-GCA-rpoB

LSC · 19169-20326 · 1158 bp

Back to Achnatherum

Pi 0.00346

MarkerSeek score 61.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 6
pi: 0.00346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1241-1247 bp	1.0	77.3
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1306-1312 bp	1.0	76.9
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.1	58.4	1305-1311 bp	1.0	76.8
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.1	58.4	1307-1313 bp	1.0	76.8
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	GTTCTTCTGCCAAGCCTTGA	50.0	50.0	58.1	58.4	1304-1310 bp	1.0	76.8