Hotspot

petN-trnC-GCA

LSC · 18171-19097 · 927 bp

Back to Achnatherum

Pi 0.00575

MarkerSeek score 53.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 8
pi: 0.00575
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	976-977 bp	1.0	71.0
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	978-979 bp	1.0	70.6
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	CAGTTCAAATCTGGGTGCCG	50.0	55.0	58.2	59.5	977-978 bp	1.0	67.6
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	AGGGGACTGCAAATCCTTTA	50.0	45.0	58.2	56.4	1001-1002 bp	1.0	66.7
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTATCCCCAGT	50.0	42.9	58.2	57.2	994-995 bp	1.0	65.4