Hotspot

ndhF-rpl32

SSC · 112016-112706 · 691 bp

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Pi 0.0145

MarkerSeek score 60.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 691
variable_sites: 10
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAAAAGGTATGATCCACGCA	AAACGCTTTTAACGATGCCC	42.9	45.0	57.9	57.7	828-848 bp	1.0	69.7
ndhF-rpl32_p2	ACAAAAGGTATGATCCACGCA	CGCTTTTAACGATGCCCAAC	42.9	50.0	57.9	58.4	825-845 bp	1.0	69.3
ndhF-rpl32_p3	ACAAAAGGTATGATCCACGCA	CCAACATCCCTTCCTCTTCC	42.9	55.0	57.9	57.6	810-830 bp	1.0	69.0
ndhF-rpl32_p4	ACAAAAGGTATGATCCACGCA	CCCAACATCCCTTCCTCTTC	42.9	55.0	57.9	57.6	811-831 bp	1.0	69.0
ndhF-rpl32_p5	AAAGAAGAAGTCCCACTGCT	AAACGCTTTTAACGATGCCC	45.0	45.0	56.7	57.7	872-892 bp	1.0	67.5