Hotspot

petB-petD

LSC · 76963-77844 · 882 bp

Back to Achimenes

Pi 0.00571

MarkerSeek score 62.70

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 5
pi: 0.00571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	TTGCCACTTCTTACTGCTGT	GATCCATCCCCCGAAAGAAC	45.0	55.0	57.4	58.0	981-987 bp	1.0	75.5
petB-petD_p2	TTGCCACTTCTTACTGCTGT	AGATCCATCCCCCGAAAGAA	45.0	50.0	57.4	58.1	982-988 bp	1.0	74.9
petB-petD_p3	ATTGCCACTTCTTACTGCTGT	GATCCATCCCCCGAAAGAAC	42.9	55.0	57.9	58.0	982-988 bp	1.0	71.5
petB-petD_p4	ATTGCCACTTCTTACTGCTGT	AGATCCATCCCCCGAAAGAA	42.9	50.0	57.9	58.1	983-989 bp	1.0	70.9
petB-petD_p5	TTGCCACTTCTTACTGCTGT	TAGATCCATCCCCCGAAAGA	45.0	50.0	57.4	57.2	983-989 bp	1.0	69.5