Hotspot

trnP-UGG-psaJ

LSC · 67346-67753 · 408 bp

Back to Achimenes

Pi 0.015
MarkerSeek score 61.80
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
408
variable_sites
6
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GCTAAAGCCCCGAACCATAG 50.0 55.0 58.1 58.4 524-531 bp 1.0 77.9
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GCTAAAGCCCCGAACCATAG 50.0 55.0 58.1 58.4 523-530 bp 1.0 77.9
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GCTAAAGCCCCGAACCATAG 50.0 55.0 58.1 58.4 535-542 bp 1.0 77.8
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GCTAAAGCCCCGAACCATAG 50.0 55.0 58.1 58.4 534-541 bp 1.0 77.8
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GCTAAAGCCCCGAACCATAG 50.0 55.0 58.1 58.4 537-544 bp 1.0 77.8