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psbE-petL

LSC · 65601-66526 · 926 bp

Back to Achimenes

Pi 0.0281

MarkerSeek score 61.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 26
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	TGATCACCACGCCTGTATTG	45.0	50.0	58.0	58.0	1516-1524 bp	1.0	81.3
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	TGATCACCACGCCTGTATTG	45.0	50.0	58.0	58.0	1515-1523 bp	1.0	81.3
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1726-1734 bp	1.0	81.2
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1725-1733 bp	1.0	81.2
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	GATCACCACGCCTGTATTGT	45.0	50.0	58.0	58.0	1514-1522 bp	1.0	81.2