Hotspot

trnT-GGU-psbD

LSC · 32172-33524 · 1353 bp

Back to Achimenes

Pi 0.00962

MarkerSeek score 64.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1353
variable_sites: 13
pi: 0.00962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1513-1521 bp	1.0	79.3
trnT-GGU-psbD_p2	ACATAGTGACCCGTTCAGGA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1561-1569 bp	1.0	79.3
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1516-1524 bp	1.0	79.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1503-1511 bp	1.0	78.3
trnT-GGU-psbD_p5	ACATAGTGACCCGTTCAGGA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1551-1559 bp	1.0	78.2