Hotspot

trnD-GUC-trnY-GUA

LSC · 31004-31111 · 108 bp

Back to Achimenes

Pi 0.0278
MarkerSeek score 68.50
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
108
variable_sites
3
pi
0.0278
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 178 bp 1.0 75.6
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 177 bp 1.0 75.6
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 199 bp 1.0 74.6
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 198 bp 1.0 74.4
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.5 58.4 232 bp 1.0 73.9