Hotspot

ycf1

SSC · 120410-125476 · 5067 bp

Back to Achillea

Pi 0.00234

MarkerSeek score 41.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5067
variable_sites: 23
pi: 0.00234
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTGTAGAGAAATTCTGCGCA	TTCACAGGCAAAAGAAGAAA	42.9	35.0	56.9	53.5	227 bp	1.0	41.8
ycf1_p2	GTTGTAGAGAAATTCTGCGCA	ATTCACAGGCAAAAGAAGAA	42.9	35.0	56.9	53.3	228 bp	1.0	41.8
ycf1_p3	GTTGTAGAGAAATTCTGCGCA	TCACAGGCAAAAGAAGAAAT	42.9	35.0	56.9	53.3	226 bp	1.0	41.8
ycf1_p4	AGTTGTAGAGAAATTCTGCGCA	TTCACAGGCAAAAGAAGAAA	40.9	35.0	58.3	53.5	228 bp	1.0	41.8
ycf1_p5	AGTTGTAGAGAAATTCTGCGC	TTCACAGGCAAAAGAAGAAA	42.9	35.0	56.6	53.5	228 bp	1.0	41.8