Hotspot

ndhG-ndhI

SSC · 115337-115695 · 359 bp

Back to Achillea

Pi 0.00743

MarkerSeek score 64.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 359
variable_sites: 5
pi: 0.00743
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAACGAAAAGGCAGAAT	TCAAATTGCTTTGGGTCGGT	45.0	45.0	57.7	58.3	559-578 bp	1.0	78.4
ndhG-ndhI_p2	TACTACTCCCAGACCCCCTA	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.7	58.3	520-539 bp	1.0	78.4
ndhG-ndhI_p3	ATACTACTCCCAGACCCCCT	TCAAATTGCTTTGGGTCGGT	55.0	45.0	58.4	58.3	521-540 bp	1.0	78.0
ndhG-ndhI_p4	AATACTACTCCCAGACCCCC	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.5	58.3	522-541 bp	1.0	77.0
ndhG-ndhI_p5	TCCCAACGAAAAGGCAGAAT	CAAATTGCTTTGGGTCGGTT	45.0	45.0	57.7	57.5	558-577 bp	1.0	76.6