Hotspot

rpl32-trnL-UAG

SSC · 109953-110836 · 884 bp

Back to Achillea

Pi 0.00679

MarkerSeek score 63.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 884
variable_sites: 12
pi: 0.00679
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCGGCATTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	988-1002 bp	1.0	83.1
rpl32-trnL-UAG_p2	GGCGGCATTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	989-1003 bp	1.0	83.1
rpl32-trnL-UAG_p3	AAAGGATATTGGGCGGCATT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	999-1013 bp	1.0	83.0
rpl32-trnL-UAG_p4	AAAGGATATTGGGCGGCATT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1000-1014 bp	1.0	83.0
rpl32-trnL-UAG_p5	GGCGGCATTAAAAGCTTTGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	998-1012 bp	1.0	82.1