Hotspot

petB-petD

LSC · 74558-75420 · 863 bp

Back to Achillea

Pi 0.00174

MarkerSeek score 60.00

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 3
pi: 0.00174
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CCGCTTCTTACTGCCGTATT	AAAAGATCGTTGGGCCATGC	50.0	50.0	58.1	59.5	1048 bp	1.0	68.0
petB-petD_p2	TATTGCCGCTTCTTACTGCC	AAAAGATCGTTGGGCCATGC	50.0	50.0	58.3	59.5	1053 bp	1.0	65.8
petB-petD_p3	CCGCTTCTTACTGCCGTATT	ACCTTTAGCCAATTTAGCCCT	50.0	42.9	58.1	57.2	1001 bp	1.0	65.7
petB-petD_p4	CCGCTTCTTACTGCCGTATT	AAAGATCGTTGGGCCATGC	50.0	52.6	58.1	58.8	1047 bp	1.0	65.3
petB-petD_p5	ACACACTTTTGTATTGCCGC	AAAAGATCGTTGGGCCATGC	45.0	50.0	57.6	59.5	1064 bp	1.0	65.2