Hotspot

petA-psbJ

LSC · 61570-62333 · 764 bp

Back to Achillea

Pi 0.00916

MarkerSeek score 65.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 11
pi: 0.00916
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	824-845 bp	1.0	69.7
petA-psbJ_p2	CGCATCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.8	57.6	875-896 bp	1.0	69.2
petA-psbJ_p3	CGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	824-845 bp	1.0	68.5
petA-psbJ_p4	TCGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	825-846 bp	1.0	68.5
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	TGTGATCGGTTTAGTAGGCG	50.0	50.0	56.8	57.4	862-883 bp	1.0	68.0