Hotspot

trnF-GAA-ndhJ

LSC · 47349-48052 · 704 bp

Back to Achillea

Pi 0.00465

MarkerSeek score 62.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 6
pi: 0.00465
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	754-807 bp	1.0	74.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	753-806 bp	1.0	74.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	761-814 bp	1.0	73.8
trnF-GAA-ndhJ_p4	AATGAGGATGCGACATCAGG	TGGATAGGATGGCCTTTACG	50.0	50.0	57.7	56.8	817-870 bp	1.0	72.8
trnF-GAA-ndhJ_p5	AATGAGGATGCGACATCAGG	GGATAGGATGGCCTTTACGT	50.0	50.0	57.7	56.8	816-869 bp	1.0	72.8