Hotspot

trnC-GCA-petN

LSC · 9304-9879 · 576 bp

Back to Achillea

Pi 0.00347

MarkerSeek score 39.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 576
variable_sites: 4
pi: 0.00347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	633-634 bp	1.0	78.6
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	685-686 bp	1.0	78.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	651-652 bp	1.0	76.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	632-633 bp	1.0	72.7
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	641-642 bp	1.0	72.6