Hotspot

rps16

Genome · 5165-6301 · 1137 bp

Back to Achatocarpus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1137
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGTGTTGGATTGGCACTACA	CCGGTTCCAAATTCGAGGAA	45.0	50.0	57.4	58.2	1260 bp	1.0	73.3
rps16_p2	TGTGTTGGATTGGCACTACA	TGACTGTCCTGACTGAACCA	45.0	50.0	57.4	58.2	1318 bp	1.0	73.1
rps16_p3	TGTGTTGGATTGGCACTACA	TACCGGTTCCAAATTCGAGG	45.0	50.0	57.4	57.6	1262 bp	1.0	71.7
rps16_p4	TGTGTTGGATTGGCACTACA	CTGACTGTCCTGACTGAACC	45.0	55.0	57.4	57.6	1319 bp	1.0	71.2
rps16_p5	TGTGTTGGATTGGCACTACA	GCATACCGGTTCCAAATTCG	45.0	50.0	57.4	57.5	1265 bp	1.0	71.1