Hotspot

matK

Genome · 2147-3664 · 1518 bp

Back to Achatocarpus

Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
Genome
length_bp
1518
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 TCAACCCTTACTGTATAAGCA CCTTGTTTTGACTGTATCGCA 38.1 42.9 54.0 56.8 1722 bp 1.0 40.0
matK_p2 TCAACCCTTACTGTATAAGCA ACCTTGTTTTGACTGTATCGC 38.1 42.9 54.0 56.8 1723 bp 1.0 40.0
matK_p3 TCAACCCTTACTGTATAAGCA CCTTGTTTTGACTGTATCGCAC 38.1 45.5 54.0 58.2 1722 bp 1.0 40.0
matK_p4 TCAACCCTTACTGTATAAGCA ACCTTGTTTTGACTGTATCGCA 38.1 40.9 54.0 58.5 1723 bp 1.0 40.0
matK_p5 TCAACCCTTACTGTATAAGCAT CCTTGTTTTGACTGTATCGCA 36.4 42.9 54.6 56.8 1722 bp 1.0 40.0