Hotspot

psaA-ycf3

Genome · 42621-43529 · 909 bp

Back to Achatocarpus

Pi 0

MarkerSeek score 62.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 909
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCGGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	659-1106 bp	1.0	82.8
psaA-ycf3_p2	TGAAAAATGACCGGGTCTGG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	659-1040 bp	1.0	82.2
psaA-ycf3_p3	TGAAAAATGACCGGGTCTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	659-1108 bp	1.0	82.0
psaA-ycf3_p4	TGAAAAATGACCGGGTCTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	659-1102 bp	1.0	81.1
psaA-ycf3_p5	TGAAAAATGACCGGGTCTGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	657-1104 bp	1.0	81.0