Hotspot

rps16-trnQ-UUG

Genome · 6302-6964 · 663 bp

Back to Achatocarpus

Pi 0
MarkerSeek score 32.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
Genome
length_bp
663
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCCTCGAATTTGGAACCGG TCTGTTTCTAAAGTTTAGTATTGG 50.0 29.2 58.2 52.8 620 bp 1.0 40.0
rps16-trnQ-UUG_p2 TGGTTCAGTCAGGACAGTCA TCTGTTTCTAAAGTTTAGTATTGG 50.0 29.2 58.2 52.8 562 bp 1.0 40.0
rps16-trnQ-UUG_p3 CCTCGAATTTGGAACCGGTA TCTGTTTCTAAAGTTTAGTATTGG 50.0 29.2 57.6 52.8 618 bp 1.0 40.0
rps16-trnQ-UUG_p4 TTCCTCGAATTTGGAACCGG TTCTGTTTCTAAAGTTTAGTATTGG 50.0 28.0 58.2 53.5 621 bp 1.0 40.0
rps16-trnQ-UUG_p5 TGGTTCAGTCAGGACAGTCA TTCTGTTTCTAAAGTTTAGTATTGG 50.0 28.0 58.2 53.5 563 bp 1.0 40.0