Hotspot

matK-trnK-UUU

Genome · 3665-4380 · 716 bp

Back to Achatocarpus

Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
Genome
length_bp
716
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCTTGCCAAGACCTATCTAGT TGGGGTTGCTAACTCAATGG 42.9 50.0 55.5 57.8 802 bp 1.0 50.3
matK-trnK-UUU_p2 TGCCAAGACCTATCTAGTTCT TGGGGTTGCTAACTCAATGG 42.9 50.0 55.5 57.8 799 bp 1.0 50.3
matK-trnK-UUU_p3 TCTTGCCAAGACCTATCTAGT GGGGTTGCTAACTCAATGGT 42.9 50.0 55.5 57.8 801 bp 1.0 50.3
matK-trnK-UUU_p4 TGCCAAGACCTATCTAGTTCT GGGGTTGCTAACTCAATGGT 42.9 50.0 55.5 57.8 798 bp 1.0 50.3
matK-trnK-UUU_p5 TCTTGCCAAGACCTATCTAGTTC TGGGGTTGCTAACTCAATGG 43.5 50.0 57.4 57.8 802 bp 1.0 49.5