Hotspot

trnH-GUG-psbA

Genome · 195-558 · 364 bp

Back to Achatocarpus

Pi 0

MarkerSeek score 34.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 364
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	433 bp	1.0	78.4
trnH-GUG-psbA_p2	ATACTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	495 bp	1.0	78.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	447 bp	1.0	78.3
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	446 bp	1.0	78.3
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	448 bp	1.0	78.3