Hotspot

ycf1

SSC · 125239-130749 · 5511 bp

Pi 0.0119

MarkerSeek score 52.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5511
variable_sites: 881
pi: 0.0119
unique_haplotype_count: 107
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGTTGTATGACCAGCGAGG	ACGGAAGAAAGGGAGGAAGA	55.0	50.0	58.1	58.0	971-1022 bp	0.970149	88.9
ycf1_p2	GAGTTGTATGACCAGCGAGG	CTCGCGACTCTTCTTTTCGA	55.0	50.0	58.1	58.0	785-809 bp	1.0	87.9
ycf1_p3	AGTTTCGCCCCTGTTTTTCT	ACGGAAGAAAGGGAGGAAGA	45.0	50.0	57.9	58.0	1014-1065 bp	0.925373	87.0
ycf1_p4	AGTTTCGCCCCTGTTTTTCT	CTCGCGACTCTTCTTTTCGA	45.0	50.0	57.9	58.0	828-852 bp	0.955224	86.1
ycf1_p5	AGTTTCGCCCCTGTTTTTCT	TCTCGCGACTCTTCTTTTCG	45.0	50.0	57.9	58.0	829-853 bp	0.955224	86.1