Hotspot

rpl22

LSC · 84567-85055 · 489 bp

Back to Acer

Pi 0.0128
MarkerSeek score 67.30
Primer available yes
Label rpl22

Feature Metadata

label_name
rpl22
feature_type
gene
region
LSC
length_bp
489
variable_sites
89
pi
0.0128
unique_haplotype_count
61
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22_p1 TTTGGCTGTGCAAACCAAAG GGGAATTTGCGCCGACTATA 45.0 50.0 58.0 58.1 622-683 bp 0.940299 88.4
rpl22_p2 TTTGGCTGTGCAAACCAAAG GCGCCGACTATAAATTTCCG 45.0 50.0 58.0 57.4 614-675 bp 1.0 87.3
rpl22_p3 GTACCAAGTCGGAACCCAAG GGGAATTTGCGCCGACTATA 55.0 50.0 58.2 58.1 583-644 bp 0.940299 87.0
rpl22_p4 TGTTGTACCAAGTCGGAACC GGGAATTTGCGCCGACTATA 50.0 50.0 57.8 58.1 587-648 bp 0.940299 86.7
rpl22_p5 TTTTTGGCTGTGCAAACCAA GGGAATTTGCGCCGACTATA 40.0 50.0 57.5 58.1 624-685 bp 0.940299 84.8