Hotspot

rpl32-trnL-UAG

SSC · 114544-115620 · 1077 bp

Back to Acer

Pi 0.0304
MarkerSeek score 74.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1077
variable_sites
319
pi
0.0304
unique_haplotype_count
111
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CCTTTCGACCGGGAATTCAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1032-1231 bp 0.932836 87.8
rpl32-trnL-UAG_p2 CCTTTCGACCGGGAATTCAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 1033-1232 bp 0.932836 87.8
rpl32-trnL-UAG_p3 CCTTTCGACCGGGAATTCAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1042-1241 bp 0.932836 86.8
rpl32-trnL-UAG_p4 CCTTTCGACCGGGAATTCAA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.2 57.8 1041-1240 bp 0.932836 86.3
rpl32-trnL-UAG_p5 CCTTTCGACCGGGAATTCAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.2 57.8 1040-1239 bp 0.932836 86.3