Hotspot

ndhF-rpl32

SSC · 113652-114390 · 739 bp

Back to Acer

Pi 0.0204
MarkerSeek score 64.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
739
variable_sites
171
pi
0.0204
unique_haplotype_count
90
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAACGAAAGGTATGATCCAAGA TTTCCAAATATTTTTACGAATACG 40.9 25.0 55.9 52.5 488-915 bp 0.955224 50.2
ndhF-rpl32_p2 ACGAAAGGTATGATCCAAGA TTTCCAAATATTTTTACGAATACG 40.0 25.0 53.8 52.5 486-913 bp 0.955224 50.2
ndhF-rpl32_p3 AACGAAAGGTATGATCCAAGA TTTCCAAATATTTTTACGAATACG 38.1 25.0 54.6 52.5 487-914 bp 0.955224 50.2
ndhF-rpl32_p4 ACGAAAGGTATGATCCAAGAA TTTCCAAATATTTTTACGAATACG 38.1 25.0 54.6 52.5 486-913 bp 0.955224 50.2
ndhF-rpl32_p5 GAACGAAAGGTATGATCCAAGAA TTTCCAAATATTTTTACGAATACG 39.1 25.0 56.6 52.5 488-915 bp 0.955224 50.2