Hotspot

ndhC-trnV-UAC

LSC · 52084-53007 · 924 bp

Back to Acer

Pi 0.0229
MarkerSeek score 64.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
924
variable_sites
216
pi
0.0229
unique_haplotype_count
85
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGAAATGCCCAGAAAATATCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 53.1 57.8 816-1001 bp 1.0 52.0
ndhC-trnV-UAC_p2 AGAAATGCCCAGAAAATATCAT AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.8 57.8 816-1001 bp 1.0 52.0
ndhC-trnV-UAC_p3 AGAAATGCCCAGAAAATATCA GAAGGTCTACGGTTCGAGTC 33.3 55.0 53.1 57.5 815-1000 bp 1.0 52.0
ndhC-trnV-UAC_p4 AGAAATGCCCAGAAAATATCA GTTCGAGTCCGTATAGCCCT 33.3 55.0 53.1 58.7 804-989 bp 1.0 52.0
ndhC-trnV-UAC_p5 ACGTTTGATATTATTAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 30.8 50.0 57.5 57.8 831-1016 bp 1.0 52.0