Hotspot

psbZ-trnG-GCC

LSC · 36777-37344 · 568 bp

Back to Acer

Pi 0.0338
MarkerSeek score 76.00
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
568
variable_sites
145
pi
0.0338
unique_haplotype_count
90
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.0 57.8 617-761 bp 0.992537 90.2
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 620-764 bp 0.992537 88.7
psbZ-trnG-GCC_p3 TCCTGATGGTTGGTCGAGTA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 613-757 bp 0.992537 88.5
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 618-762 bp 0.992537 88.3
psbZ-trnG-GCC_p5 TTCTCCTGATGGTTGGTCGA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.4 57.8 616-760 bp 0.992537 87.5