Hotspot

trnM-CAU-psbD

LSC · 31966-33468 · 1503 bp

Back to Acer

Pi 0.0132
MarkerSeek score 65.10
Primer available yes
Label trnM-CAU-psbD

Feature Metadata

label_name
trnM-CAU-psbD
feature_type
igs
region
LSC
length_bp
1503
variable_sites
257
pi
0.0132
unique_haplotype_count
98
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-psbD_p1 GTGGTAGAGTAACGCCATGG TCTCCGTAACCAGTCATCCA 55.0 50.0 58.1 57.8 1587-1649 bp 1.0 89.7
trnM-CAU-psbD_p2 TCAGTGGTAGAGTAACGCCA TCTCCGTAACCAGTCATCCA 50.0 50.0 58.1 57.8 1590-1652 bp 1.0 89.5
trnM-CAU-psbD_p3 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1618-1680 bp 1.0 89.4
trnM-CAU-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1621-1683 bp 1.0 89.2
trnM-CAU-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1620-1682 bp 1.0 89.0