Hotspot

rpoB-trnC-GCA

LSC · 27066-28230 · 1165 bp

Back to Acer

Pi 0.0133
MarkerSeek score 65.30
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1165
variable_sites
187
pi
0.0133
unique_haplotype_count
93
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TAGACATTCCCGCATTTCCG AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.1 58.4 1034-1274 bp 0.985075 88.1
rpoB-trnC-GCA_p2 TAGACATTCCCGCATTTCCG TTGTTAATCAGGCGACACCC 50.0 50.0 58.1 58.2 1070-1310 bp 0.940299 87.7
rpoB-trnC-GCA_p3 TAGACATTCCCGCATTTCCG GCGACACCCAGATTTGAACT 50.0 50.0 58.1 58.5 1059-1299 bp 0.962687 86.3
rpoB-trnC-GCA_p4 TAGACATTCCCGCATTTCCG TAATCAGGCGACACCCAGAT 50.0 50.0 58.1 58.5 1066-1306 bp 0.932836 84.9
rpoB-trnC-GCA_p5 TAGACATTCCCGCATTTCCG GTTAATCAGGCGACACCCAG 50.0 55.0 58.1 58.6 1068-1308 bp 0.940299 84.2