Hotspot

atpH-atpI

LSC · 14069-15216 · 1148 bp

Back to Acer

Pi 0.015
MarkerSeek score 64.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1148
variable_sites
237
pi
0.015
unique_haplotype_count
97
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACAGAAGCGGCAGAAATCA TTTTTGCAACTTTAGCGGCG 45.0 45.0 58.1 58.5 1169-1270 bp 1.0 87.0
atpH-atpI_p2 AACAGAAGCGGCAGAAATCA ACAAGCGGTATTCAAGCTCT 45.0 45.0 58.1 57.2 1191-1292 bp 1.0 85.0
atpH-atpI_p3 ACAGAAGCGGCAGAAATCAG TTTTTGCAACTTTAGCGGCG 50.0 45.0 58.6 58.5 1168-1269 bp 1.0 83.4
atpH-atpI_p4 AGAAGCGGCAGAAATCAGTG TTTTTGCAACTTTAGCGGCG 50.0 45.0 58.6 58.5 1166-1267 bp 1.0 83.4
atpH-atpI_p5 CAGAAGCGGCAGAAATCAGT TTTTTGCAACTTTAGCGGCG 50.0 45.0 58.6 58.5 1167-1268 bp 1.0 83.4