Hotspot

rps16-trnQ-UUG

LSC · 6365-7453 · 1089 bp

Back to Acer

Pi 0.016
MarkerSeek score 64.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1089
variable_sites
237
pi
0.016
unique_haplotype_count
92
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 583-1216 bp 1.0 90.3
rps16-trnQ-UUG_p2 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 598-1231 bp 1.0 88.4
rps16-trnQ-UUG_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 597-1230 bp 1.0 88.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 587-1220 bp 1.0 85.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 613-1246 bp 1.0 85.4