Hotspot

trnH-GUG-psbA

LSC · 87-476 · 390 bp

Back to Acer

Pi 0.0279
MarkerSeek score 67.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
390
variable_sites
123
pi
0.0279
unique_haplotype_count
103
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 403-614 bp 0.992537 90.4
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 402-613 bp 0.992537 90.4
trnH-GUG-psbA_p3 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 404-615 bp 0.992537 90.4
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 389-600 bp 0.985075 90.1
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 342-553 bp 0.985075 89.8