Hotspot

petA-psbJ

LSC · 62727-63505 · 779 bp

Back to Acanthus

Pi 0.0603

MarkerSeek score 80.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 63
pi: 0.0603
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	CTGGACGGATTCCTCTTTGG	50.0	55.0	58.0	58.0	948-1122 bp	1.0	88.9
petA-psbJ_p2	TTTATCCCGACGGAAGCAAG	CTGGACGGATTCCTCTTTGG	50.0	55.0	58.0	58.0	1098-1272 bp	1.0	88.7
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	CTTAGCCCATTCGGAAGGAC	50.0	55.0	58.0	58.0	1056-1230 bp	1.0	88.5
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1208-1382 bp	1.0	88.1
petA-psbJ_p5	CAGGGCCAGAACTTCTTGTT	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1447-1621 bp	1.0	87.7