Hotspot

accD-psaI

LSC · 58806-59533 · 728 bp

Back to Acanthus

Pi 0.0535

MarkerSeek score 81.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 52
pi: 0.0535
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AATTCCATCAAAGCGTACTA	GCCGGAAATACTAGGCCTAC	35.0	55.0	52.3	57.5	808 bp	0.333333	13.3
accD-psaI_p2	ATTCCATCAAAGCGTACTAA	GCCGGAAATACTAGGCCTAC	35.0	55.0	52.3	57.5	807 bp	0.333333	13.3
accD-psaI_p3	TAATTCCATCAAAGCGTACT	GCCGGAAATACTAGGCCTAC	35.0	55.0	52.3	57.5	809 bp	0.333333	13.3
accD-psaI_p4	AATTCCATCAAAGCGTACTAA	GCCGGAAATACTAGGCCTAC	33.3	55.0	53.2	57.5	808 bp	0.333333	13.3
accD-psaI_p5	TAATTCCATCAAAGCGTACTA	GCCGGAAATACTAGGCCTAC	33.3	55.0	52.4	57.5	809 bp	0.333333	13.3