Hotspot

psaA-ycf3

LSC · 41673-42322 · 650 bp

Back to Acanthus

Pi 0.0874

MarkerSeek score 84.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 76
pi: 0.0874
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AATCATGAGCATCGGCATGT	ATTGCAGAGGCTTGGTTTGA	45.0	45.0	58.0	58.0	933-1079 bp	1.0	88.9
psaA-ycf3_p2	AATCATGAGCATCGGCATGT	TTGCAGAGGCTTGGTTTGAT	45.0	45.0	58.0	58.0	932-1078 bp	1.0	88.9
psaA-ycf3_p3	GATCACTTAGCCACGCTTCA	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	58.0	58.0	1080-1226 bp	1.0	88.0
psaA-ycf3_p4	GATCACTTAGCCACGCTTCA	TTGCAGAGGCTTGGTTTGAT	50.0	45.0	58.0	58.0	1079-1225 bp	1.0	88.0
psaA-ycf3_p5	GATCACTTAGCCACGCTTCA	TGAAATTGCAGAGGCTTGGT	50.0	45.0	58.0	58.0	1084-1230 bp	1.0	87.9