Hotspot

trnS-UGA-trnG-GCC

LSC · 35511-36297 · 787 bp

Back to Acanthus

Pi 0.0507

MarkerSeek score 81.80

Primer available yes

Label trnS-UGA-trnG-GCC

Feature Metadata

label_name: trnS-UGA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 51
pi: 0.0507
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-trnG-GCC_p1	GTTTTCAAGACCGGGGCTAT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.9	58.1	809-886 bp	1.0	87.3
trnS-UGA-trnG-GCC_p2	GTTTTCAAGACCGGGGCTAT	GAATCGAACCCGCATCTTCT	50.0	50.0	57.9	58.1	810-887 bp	1.0	87.3
trnS-UGA-trnG-GCC_p3	TTTTCAAGACCGGGGCTATC	AATCGAACCCGCATCTTCTC	50.0	50.0	57.6	58.1	808-885 bp	1.0	85.0
trnS-UGA-trnG-GCC_p4	TTTTCAAGACCGGGGCTATC	GAATCGAACCCGCATCTTCT	50.0	50.0	57.6	58.1	809-886 bp	1.0	85.0
trnS-UGA-trnG-GCC_p5	CTATCAACCGCTCAGCCATC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.5	58.1	793-870 bp	1.0	84.5