Hotspot

atpH-atpI

LSC · 13478-14488 · 1011 bp

Back to Acanthus

Pi 0.0729

MarkerSeek score 84.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1011
variable_sites: 105
pi: 0.0729
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TGGCCCAAAGAAACGAAAGA	TGCTTTGGCTTTACTCACGT	45.0	45.0	57.9	58.0	1994-2532 bp	1.0	87.6
atpH-atpI_p2	GATGCCCTCTACAGCTTGAC	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.1	58.0	1459-1987 bp	1.0	86.7
atpH-atpI_p3	GATGCCCTCTACAGCTTGAC	GGCAAATAGGGGGTTTCCAA	55.0	50.0	58.1	58.1	1790-2318 bp	1.0	85.7
atpH-atpI_p4	GATGCCCTCTACAGCTTGAC	CAAACCATTCCGACTAGGGG	55.0	55.0	58.1	58.0	1689 bp	0.333333	52.6
atpH-atpI_p5	TGGCCCAAAGAAACGAAAGA	CAAACCATTCCGACTAGGGG	45.0	55.0	57.9	58.0	2224 bp	0.333333	52.5