Hotspot

trnS-GCU-trnG-UCC

LSC · 8130-8844 · 715 bp

Back to Acanthus

Pi 0.0808

MarkerSeek score 84.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 80
pi: 0.0808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	61.1	45.0	56.4	54.0	753-927 bp	1.0	51.8
trnS-GCU-trnG-UCC_p2	CCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	61.1	38.1	56.4	55.5	767-941 bp	1.0	51.7
trnS-GCU-trnG-UCC_p3	CCACTCAGCCATCTCTCC	AACGAATCACACTTTTACCACT	61.1	36.4	56.4	56.2	768-942 bp	1.0	51.7
trnS-GCU-trnG-UCC_p4	CCACTCAGCCATCTCTCC	AAACGAATCACACTTTTACCACT	61.1	34.8	56.4	56.9	769-943 bp	1.0	51.7
trnS-GCU-trnG-UCC_p5	CCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCAC	61.1	40.0	56.4	53.9	767-941 bp	1.0	51.7