Hotspot

rps16-trnQ-UUG

LSC · 5740-6851 · 1112 bp

Back to Acanthus

Pi 0.0566

MarkerSeek score 84.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 89
pi: 0.0566
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1185-1272 bp	1.0	88.1
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1189-1276 bp	1.0	83.4
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1177-1264 bp	1.0	83.0
rps16-trnQ-UUG_p4	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	1184-1271 bp	1.0	82.4
rps16-trnQ-UUG_p5	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	1180-1267 bp	1.0	80.8