Hotspot

ycf1

SSC · 121783-127395 · 5613 bp

Back to Acanthophyllum

Pi 0.00356

MarkerSeek score 43.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 20
pi: 0.00356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCGCGATATTGAAGGGCA	TCCAACTTCCAGAGTGGTCT	45.0	50.0	58.0	57.9	1170 bp	1.0	79.7
ycf1_p2	TTTCGCGATATTGAAGGGCA	CCCGAGAACGACCTTCTTTT	45.0	50.0	58.0	57.8	885 bp	1.0	79.4
ycf1_p3	TTTCGCGATATTGAAGGGCA	ATTCCTTTTGGTTCCTCCCG	45.0	50.0	58.0	57.8	901 bp	1.0	79.0
ycf1_p4	TTTCGCGATATTGAAGGGCA	TTTTGGTTCCTCCCGAGAAC	45.0	50.0	58.0	57.7	896 bp	1.0	78.6
ycf1_p5	TGTCGGATTCATGAATCGGG	TCCAACTTCCAGAGTGGTCT	50.0	50.0	57.8	57.9	617 bp	1.0	78.2