Hotspot

rpl16

LSC · 80393-81702 · 1310 bp

Back to Acanthophyllum

Pi 0.00619

MarkerSeek score 50.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1310
variable_sites: 8
pi: 0.00619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCGACGATTGCTAGCTCCTA	AATACAAATAGCAGGCCGCA	50.0	45.0	58.0	57.9	1829-1851 bp	1.0	80.7
rpl16_p2	GGGCAATTGCACCAAAAACT	AATACAAATAGCAGGCCGCA	45.0	45.0	58.0	57.9	2046-2068 bp	1.0	80.6
rpl16_p3	GGGCAATTGCACCAAAAACT	TCGAAGGAATTGCACGCATA	45.0	45.0	58.0	58.0	2381 bp	0.5	59.6
rpl16_p4	TCGACGATTGCTAGCTCCTA	TCGAAGGAATTGCACGCATA	50.0	45.0	58.0	58.0	2164 bp	0.5	59.6
rpl16_p5	AACATCGCCAATACGAGCAT	TCGAAGGAATTGCACGCATA	45.0	45.0	58.0	58.0	2185 bp	0.5	59.5