Hotspot

petD

LSC · 75717-76966 · 1250 bp

Back to Acanthophyllum

Pi 0.00656

MarkerSeek score 51.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1250
variable_sites: 8
pi: 0.00656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTCCTTACCTCGGGACCAA	TGCTCTATTCATGCCGGTTC	50.0	50.0	58.0	58.0	2322-2349 bp	1.0	80.8
petD_p2	TCCTTACCTCGGGACCAAAT	TGCTCTATTCATGCCGGTTC	50.0	50.0	58.0	58.0	2320-2347 bp	1.0	80.8
petD_p3	AGGCTTTCGCTTCTGTTCAA	TGCTCTATTCATGCCGGTTC	45.0	50.0	58.0	58.0	2538-2565 bp	1.0	80.7
petD_p4	GGTCCGCAAGTATGATGGTT	TGCTCTATTCATGCCGGTTC	50.0	50.0	58.0	58.0	2466-2493 bp	1.0	80.7
petD_p5	AAACCGCGCGAATTAACTTG	TGCTCTATTCATGCCGGTTC	45.0	50.0	58.0	58.0	2395-2422 bp	1.0	80.7