Hotspot

trnT-GGU-psbD

LSC · 30887-31726 · 840 bp

Back to Acanthophyllum

Pi 0.00477

MarkerSeek score 61.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 4
pi: 0.00477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACGGTCTCTACGTAACCAGT	55.0	50.0	58.1	57.8	979-995 bp	1.0	78.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.1	57.8	982-998 bp	1.0	78.3
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.3	57.8	956-972 bp	1.0	76.7
trnT-GGU-psbD_p4	TGGTAGAGTAACGCCATGGT	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.4	57.8	978-994 bp	1.0	75.5
trnT-GGU-psbD_p5	CTCAGTGGTAGAGTAACGCC	ACGGTCTCTACGTAACCAGT	55.0	50.0	57.4	57.8	983-999 bp	1.0	74.4