Hotspot

atpF-atpH

LSC · 12498-12973 · 476 bp

Back to Acanthophyllum

Pi 0.00436

MarkerSeek score 62.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 2
pi: 0.00436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AGTGACCCAAGAAAGCGAAA	GATTGGTTGTCGCATTAGCC	45.0	50.0	57.7	57.5	546-563 bp	1.0	76.2
atpF-atpH_p2	AGTGACCCAAGAAAGCGAAA	GGATTGGTTGTCGCATTAGC	45.0	50.0	57.7	57.5	547-564 bp	1.0	76.2
atpF-atpH_p3	CAGTGACCCAAGAAAGCGAA	GATTGGTTGTCGCATTAGCC	50.0	50.0	58.4	57.5	547-564 bp	1.0	75.7
atpF-atpH_p4	CAGTGACCCAAGAAAGCGAA	GGATTGGTTGTCGCATTAGC	50.0	50.0	58.4	57.5	548-565 bp	1.0	75.7
atpF-atpH_p5	AGTGACCCAAGAAAGCGAAA	CCTGAGGCAGAGGGAAAAAT	45.0	50.0	57.7	57.5	619-636 bp	1.0	75.5