Hotspot

trnR-UCU-atpA

LSC · 9531-9651 · 121 bp

Back to Acanthophyllum

Pi 0.0168

MarkerSeek score 62.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 2
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TGGAACGCTTTCTACTTCAGG	47.6	47.6	57.3	58.0	176 bp	1.0	60.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	GGAACGCTTTCTACTTCAGGA	47.6	47.6	57.3	57.7	175 bp	1.0	57.8
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	TGGAACGCTTTCTACTTCAGG	47.6	47.6	56.9	58.0	211 bp	1.0	56.8
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACATTTACTGAGGAAGCGGA	47.6	45.0	57.3	56.6	228 bp	1.0	56.3
trnR-UCU-atpA_p5	GGAATGAAATGAAAAGCGTCCA	TGGAACGCTTTCTACTTCAGG	40.9	47.6	57.8	58.0	241 bp	1.0	55.6