Hotspot

ndhK

LSC · 49705-50097 · 393 bp

Pi 0.0102

MarkerSeek score 45.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 393
variable_sites: 4
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	CCTCGTGTCACCAGTTCAAA	AGAACCAATGGGGATGCTTG	50.0	50.0	58.1	58.1	903-904 bp	1.0	79.4
ndhK_p2	CCTCGTGTCACCAGTTCAAA	GAGAACCAATGGGGATGCTT	50.0	50.0	58.1	57.9	904-905 bp	1.0	79.4
ndhK_p3	CAAAGGAACCCTGCCTGATT	AGAACCAATGGGGATGCTTG	50.0	50.0	58.1	58.1	657 bp	1.0	79.4
ndhK_p4	CCTCGTGTCACCAGTTCAAA	GGGGATGCTTGGGTACAATT	50.0	50.0	58.1	57.8	894-895 bp	1.0	79.3
ndhK_p5	CAAAGGAACCCTGCCTGATT	GAGAACCAATGGGGATGCTT	50.0	50.0	58.1	57.9	658 bp	1.0	79.3