Hotspot

ndhG

SSC · 115236-115518 · 283 bp

Pi 0.0142

MarkerSeek score 46.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 283
variable_sites: 4
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AATACATCCGTGGGACAAGC	GCCACAAGGTACATTGGACA	50.0	50.0	58.0	58.1	887-1137 bp	1.0	80.0
ndhG_p2	GGACAAGCGGATTCACATCT	GCCACAAGGTACATTGGACA	50.0	50.0	58.0	58.1	986-1236 bp	1.0	79.9
ndhG_p3	AGCTCGAACACATTGGGTAC	GCCACAAGGTACATTGGACA	50.0	50.0	57.9	58.1	870-1120 bp	1.0	79.5
ndhG_p4	GGACAAGCGGATTCACATCT	TGAGCCACAAGGTACATTGG	50.0	50.0	58.0	57.8	989-1239 bp	1.0	79.1
ndhG_p5	GGACAAGCGGATTCACATCT	AGCCACAAGGTACATTGGAC	50.0	50.0	58.0	57.8	987-1237 bp	1.0	79.1