Hotspot

ndhD

SSC · 113546-114419 · 874 bp

Pi 0.0116

MarkerSeek score 60.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 874
variable_sites: 10
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCATTGCACAACGAAGAATC	GCACGGGCTTTTCTGGTATA	40.0	50.0	54.6	58.0	997-1006 bp	1.0	54.3
ndhD_p2	TTCATTGCACAACGAAGAATC	GCACGGGCTTTTCTGGTATA	38.1	50.0	55.4	58.0	998-1007 bp	1.0	52.8
ndhD_p3	TTTCATTGCACAACGAAGAA	GCACGGGCTTTTCTGGTATA	35.0	50.0	54.4	58.0	999-1008 bp	1.0	52.1
ndhD_p4	TTTTCATTGCACAACGAAGA	GCACGGGCTTTTCTGGTATA	35.0	50.0	54.4	58.0	1000-1009 bp	1.0	52.1
ndhD_p5	TTTTCATTGCACAACGAAGAA	GCACGGGCTTTTCTGGTATA	33.3	50.0	55.2	58.0	1000-1009 bp	1.0	50.8